PMC

Representative light microscopic micrographs (left) and height profiles (xz scans, right) determined by confocal microscopy of Cetpyrsal/PTX coating (top), HA/PTX coating (middle) and PVP/PTX coating (bottom). The indicated thickness ± SD has been averaged over 8 positions along the PEBAX tube fitted to a stainless steel pin with ten measurement points each (right).

(A) Schematic diagram of trehalose coating procedure. (B) The TMB assay, six days after coating, the proteins were denatured in the absence of trehalose coating. Secondary trehalose coating preserved the protein structure. (C) Spectrophotometry of the TMB solutions. Without trehalose coating, proteins on the Al plate were denatured after 6 days. In the presence of trehalose coating, the protein was still not denatured twelve days after coating. Asterisks (* and **) denote statistical significance of the differences in colorimetric intensity of surface-immobilized ABP-Cn-AFP_G124Y with and without trehalose over the time course (p < 0.01, paired t-test, n = 4). Error bars indicate standard deviation.

a 7 nm coating hysteresis cycle. b 14 nm coating hysteresis cycle. c 20 nm coating hysteresis cycle. d 33 nm coating hysteresis cycle. e 70 nm coating hysteresis cycle. f ΔR/R for each of the coating thicknesses. The red highlighted area indicated coatings that are more suitable for consistent conduction performance over strain, including the 20, 33, and 70 nm coatings. The inset table lists the tabulated hysteresis integration areas, indicating the most (33 nm) and least (7 nm) consistent conduction performance over the single cycle.

Mean in vitro daily release of mifepristone from intravaginal rings containing SD.
Mean in vitro daily release of mifepristone from sustained intravaginal rings containing solid dispersions (SD, 1:0.5, wdrug/wpvp) of mifepristone with different concentration of coating solutions (n=3) over the first 7 days. The initial burst release of mifepristone from the IVR devices with coating was observed to be 0.5 times of the IVR devices without coating (5.40 mg). (-♦-) without coating, (-▪-) 10% coating solution, (-▴-) 18% coating solution, (-×-) 25% coating solution, (-Δ-) 30% coating solution, (-▫-) 50% coating solution.